Pan Cytokeratin Plus 1,0 mL
Specificity and Comments:
AE1/AE3 recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa.
Clone 5DT recognizes cytokeratin (CK) 8 and 18 intermediate filament proteins. These are 52.5 kD and 45kD respectively. In normal tissues, 5DT recognizes all simple and glandular epithelium. In the past, AE1/AE3 has had problems marking certain tissues types and adenocarcinomas. The addition of CK 8/18 remedies some of these problems. For example a study of twenty-eight lipid cell (steroid cell) tumors of the ovary were studied by immunohistochemistry; 46% were positive for Cytokeratin 8/18 antibody, 37% were positive with the Cytokeratin cocktail AE1/AE3.
Clone: AE1/AE3 + 5D3
Isotypes: IgG1
Species Reactivity:
Human, dog, fish, mouse, rat, rabbit, bovine and chicken
Known Applications: Immunohistochemistry (frozen and formalin-fixed/paraffin embedded)
Positive Control: Skin or adenocarcinoma
Cellular Localization: Cytoplasmic
Supplied As:
Buffer with protein carrier and preservative
Storage and Stability:
Store vial at 4°C. Stable for 18 months.